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Subject: INSTRUCTIONS FOR EXAM OF SAMPLES FM GULF SUSPECTED OF AGENTS    

Unit: OTSG        

Parent Organization: HSC         

Box  ID: BX003201

Folder Title: TRIP REPORT TO ENGLAND                                                                          

Document Number:          5

Folder Seq  #:          2








          If sample is a swab and is positive by CAM proceed as follows:

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                 IPC>IRW31c>kq 'A.S

          Remove swab from containing tube and place in 10 ml phosphate
          buffered saline; agitate vigorously on vortex mixer for 10-15
          seconds.
                                      s@ension

          Loop-full into                copic
          5 ml blood                    ation
          incubate 4 h at               ing Gram stain
                                         nofluorescence

          Make up volume to
          15 ml with PBS                                    ELISA test


          Biotrace examination

          Macfadyean's test
          Fibrinolysin test
          Culture on:
          BCGA overnight
          PLET 48 h               Select colonies
          NA overnight
          MacConkey overnight

                                        API tests:
                                        20E
                                        20E rapid
                                        50

          Treatment of sample for chemical analysis
          1.  Place about I g of the sample in a 3ml "Reactivial"
          2.  Add 1 ml of dichloromethane (methylene chloride). Cap
              with PTFE-lined septum and shake for 30 sec. Add 1 ml of
              10% formaldehyde through the septum with a syringe and
              shake for 30 sec.
          3.  Decontaminate exterior of "Reactivial" by wiping with
              muslin soaked in 10% hypochlorite solution.
          4.  Transfer "Reactivial" to charcoal-filled CW agent
              container. Wipe outside of container with 10%
              hypochlorite solution.
          5.  Take the container to CDD.


          RESULTS

          The results should be reported on the NBC6 Form (Annex B).

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Document 17 f:/Week-36/BX003201/TRIP REPORT TO ENGLAND/instructions for exam of samples fm gulf suspect:12179609283942
Control Fields 17
File Room = sep96_declassified
File Cabinet = Week-36
Box ID = BX003201
Unit = OTSG
Parent Organization = HSC
Folder Title = TRIP REPORT TO ENGLAND
Folder Seq # = 2
Subject = INSTRUCTIONS FOR EXAM OF SAMPLES FM GULF SUSPECT
Document Seq # = 5
Document Date =
Scan Date =
Queued for Declassification = 01-JAN-1980
Short Term Referral = 01-JAN-1980
Long Term Referral = 01-JAN-1980
Permanent Referral = 01-JAN-1980
Non-Health Related Document = 01-JAN-1980
Declassified = 17-DEC-1996