INSTRUCTIONS FOR EXAM OF SAMPLES FM GULF SUSPECTED OF AGENTS

File: 123096_sep96_decls41_0008.txt
Page: 0008
Total Pages: 17

Subject: INSTRUCTIONS FOR EXAM OF SAMPLES FM GULF SUSPECTED OF AGENTS    

Unit: OTSG        

Parent Organization: HSC         

Box  ID: BX003201

Folder Title: TRIP REPORT TO ENGLAND                                                                          

Document Number:          5

Folder Seq  #:          2







            1. If sample appears to be contaminated by diluted chemical
            agent as indicated by CAM proceed as follows:



                                     Aqueous sample

            Make ring of petroleum jelly on slide. Place drop of sample
            within ring, cover with coverslip, examine microscopically for
            presence of microorganisms by  P4 hase contrast.
            If no organisms are seen, centrifuge sample in Eppendorf tube.
            Examine deposit microscopically for organisms. If none seen
            proceed as follows:
                               deposit
            Loop-full of deposit     @direct microscopic
            into 5 ml blood             examination including
            incubate 4 h at 37C         Gtam stain and
                                         immunofluorescence
            Make volume up to@
            15 ml with PBS
                                                            ELISA test
            Biotrace examination

            Macfadyean's test
            Fibrinolysin test
            culture on:
            BAB + haemin overnight)
            PLET 48 h              )-@Select colonies
            NA overnight
            MacConkey overnight
                                          API tests:
                                          20E
                                          20B rapid
                                          50
            Treatment of sample for chemical ana
            1.  Place about 1 g of the sample in a 3ml "Reactivial"
            2.  Add 1 ml of dichloromethane (methylene chloride). Cap
                with PTFE-lined septum and shake for 30 sec. Add 1 ml of
                10% formaldehyde through the septum with a syringe and
                shake for 30 sec.
            3.  Decontaminate exterior of "Reactivial" by wiping with
                 muslin soaked in 10% hypochlorite solution.
            4.  Transfer "Reactivial" to charcoal-filled CW agent
ontainer with 10%
                hypochlorite solution.
            5.  Take the container to CDD.
            RESULTS
            The results should be reported on the NBC6 Form (Annex B).

Document 17 f:/Week-36/BX003201/TRIP REPORT TO ENGLAND/instructions for exam of samples fm gulf suspect:12179609283942
Control Fields 17
File Room = sep96_declassified
File Cabinet = Week-36
Box ID = BX003201
Unit = OTSG
Parent Organization = HSC
Folder Title = TRIP REPORT TO ENGLAND
Folder Seq # = 2
Subject = INSTRUCTIONS FOR EXAM OF SAMPLES FM GULF SUSPECT
Document Seq # = 5
Document Date =
Scan Date =
Queued for Declassification = 01-JAN-1980
Short Term Referral = 01-JAN-1980
Long Term Referral = 01-JAN-1980
Permanent Referral = 01-JAN-1980
Non-Health Related Document = 01-JAN-1980
Declassified = 17-DEC-1996