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Subject: INSTRUCTIONS FOR EXAM OF SAMPLES FM GULF SUSPECTED OF AGENTS    

Unit: OTSG        

Parent Organization: HSC         

Box  ID: BX003201

Folder Title: TRIP REPORT TO ENGLAND                                                                          

Document Number:          5

Folder Seq  #:          2








           If sample is Positive by CAM and consists of particulate
           material other than soil e.g. paint scrapings etc. proceed as
           follows:


                  ite-U!ATH IPOIRU!3clDN 40]F S@IRTI@


           Place small quantity of material in test tube  add 10 ml of
           PBS.
           Agitate vigorously on vortex mixer for 10-15 seconds.
           Filter sample through 2 layers of gauze to remove large
           particles.                      I
                                      Suspension

           Loop-fUll into                copic
           5 ml blood                  ination
           incubate 4 h a              uding Gram stain
                                       immunofluorescence

           Make up volume
           15 ml with PBS                                    ELISA test
           Biotra;e examination
           Macfad@ean's test
           Fibrinolysin test
           Culture on:
           BCGA overnight
           PLET 48 h               Select colonies
           NA overnight
           MacConkey overnight
                                         API tests:
                                         20E
                                         20E rapid
                                         50
           Treatment of sample for chemical analvsis
           1.  Place about 1 g of the sample in a 3ml "Reactivial"
           2.  Add 1 ml of dichloromethane (methylene chloride). Cap
               with PTFE-lined septum and shake for 30 sec. Add I ml of
               10% formaldehyde through the septum with a syringe and
               shake for 30 sec.
           3.  Decontaminate exterior of "Reactivial" by wiping with
                muslin soaked in 10% hypochlorite solution.
           4.  Transfer "Reactivial" to charcoal-filled CW agent
               container. Wipe outside of container with 10%
               hypochlorite solution.
           5.  Take the container to ODD.


           RESULTS
 the NBC6 Form (Annex B).

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Document 17 f:/Week-36/BX003201/TRIP REPORT TO ENGLAND/instructions for exam of samples fm gulf suspect:12179609283942
Control Fields 17
File Room = sep96_declassified
File Cabinet = Week-36
Box ID = BX003201
Unit = OTSG
Parent Organization = HSC
Folder Title = TRIP REPORT TO ENGLAND
Folder Seq # = 2
Subject = INSTRUCTIONS FOR EXAM OF SAMPLES FM GULF SUSPECT
Document Seq # = 5
Document Date =
Scan Date =
Queued for Declassification = 01-JAN-1980
Short Term Referral = 01-JAN-1980
Long Term Referral = 01-JAN-1980
Permanent Referral = 01-JAN-1980
Non-Health Related Document = 01-JAN-1980
Declassified = 17-DEC-1996