APPENDIX D SOP COLLECT OF PERIPHERAL BLOOD FOR BIO SURVIEL

File: 091896_aug96_decls42_0004.txt
Page: 0004
Total Pages: 4

Subject: APPENDIX D  SOP COLLECT OF PERIPHERAL BLOOD FOR BIO SURVIEL     

Box  ID: BX005154

Document Number:         21

Folder Title: PREVENTIVE MEDICINE-ENVIRONMENTAL THREAT-USAEHA                                                 

Folder Seq #:         71

Unit: 22D SUPCOM  

Parent Organzation: ARCENT      





               APPENDIX D:       Blood  Coll2ction and Fractionation             Page 4 of 4
               Plan tor the Biologic Surveillance Initiative, Kuwait Oil Fires Health Risk
               AS3&33ment.

                   f.  RoLnove packed RSCO by carefully inserting a 14 gauge 4 inch pipetting
               needle (on 30ml syringe) through the edge of the buffy coat to the bottom of
               the tube. Slowly withdraw most of the RBCS, leaving 8  ml of blood in tube.
               This remaining 8 ml will contain the buffy coat, plasma and about Bmlo of
               RECS. Save 13mlB of withdrawn RECs in a 15ml scrowtop polypropylene
               centrifuge tube (as above). Do no overfill tubes as they may burst with
               freezing.

                   g. You now should have two 15 ml tubeas    sera and RBCS) and one 50 ml
               tube (buffy coat) for each individual. Store frozen (-70C or dry ice, if not
               available -20C will do).

Document 4 f:/Week-34/BX005154/PREVENTIVE MEDICINE-ENVIRONMENTAL THREAT-USAEHA/appendix d sop collect of peripheral blood for :09139616151390
Control Fields 17
File Room = aug96_declassified
File Cabinet = Week-34
Box ID = BX005154
Unit = 22D SUPCOM
Parent Organization = ARCENT
Folder Title = PREVENTIVE MEDICINE-ENVIRONMENTAL THREAT-USAEHA
Folder Seq # = 71
Subject = APPENDIX D SOP COLLECT OF PERIPHERAL BLOOD FOR
Document Seq # = 90
Document Date =
Scan Date = 30-AUG-1996
Queued for Declassification = 01-JAN-1980
Short Term Referral = 01-JAN-1980
Long Term Referral = 01-JAN-1980
Permanent Referral = 01-JAN-1980
Non-Health Related Document = 01-JAN-1980
Declassified = 13-SEP-1996