APPENDIX D SOP COLLECT OF PERIPHERAL BLOOD FOR BIO SURVIEL

File: 091896_aug96_decls42_0003.txt
Page: 0003
Total Pages: 4

Subject: APPENDIX D  SOP COLLECT OF PERIPHERAL BLOOD FOR BIO SURVIEL     

Box  ID: BX005154

Document Number:         21

Folder Title: PREVENTIVE MEDICINE-ENVIRONMENTAL THREAT-USAEHA                                                 

Folder Seq #:         71

Unit: 22D SUPCOM  

Parent Organzation: ARCENT      




               APPENDIX D:        Blood Collection and Fractionation                   P2ge 3 of 4
               Plan tor the Biologic Surveillance Initiative, Kuwait Oil Fires Health Risk
               Assessment.
                                             DEPARTMENT OF THE ARMY
                                    U.S. ARMY ENVIRONMENTAL HYGIENE AGENCY
                                                  EDGEWOOD AREA
                                    ABERDEEN PROVING GROUND, MARYLAND 21010


               liSHB-HO-R                                                        29 JULY,1991


                                          STANDARD OPERATING PROCEDURE
                                                        FOR
            COLLECTION OF BUFFY COAT (BLOOD FRACTIONATION PROTOCOL) FOR BIOLOGIC SURVEILLANCE
                                              TEAM, KUWAIT OIL FIRES

               1. PURPOSE: To establish guidelines for the proper procedures to follow when
               fractionating blood from individuals being monitored for exposure to the
               Kuwait oil fires.

               2. SCOPE: This SOP applies to all military and c4vilian personnel who are
               exposed to smokes from the Kuwait oil fires and ar; under surveillance for
               said exposure.

               3.  REFERENCE:
                   a.   AR 40-5, Preventive Medlcine
                   b.   FM B-230 Medical Specialist
                   c.   TB MED 503 The Industrial Hygiene Progran
                   d.   FM 8-273-1 Nursing Skills For Allied Health Services
                   e.   Blood Fractionation Protocol from Johns Hopkins University

               4. RESPONSIBILITIES: This program is designed to enable the surveillance
               Team staff to:
                   a.   Perform medical surveillance for exposure to Kuwait oil fire smoke.
                   b.   Properly label all samples and questionnaires for surveillance.
que.
                   d.   Handle and store all samples in a manner that is conducive with
               further  testing and minimizes any deterioration of each sa-nple.


               5. GENERAL:


                   a. Draw 40-45 mls of blood in three 15ril vacutainer tubes containing
               heparin(green tops). Invert tubes occasionally until processing is
               accomplished.

                   b. Combine all three 15ml tubes into one 50,nl centrifuge tube (Corning
               125330).

                   C. Centrifuge at 10OOg (20OOrpm in counter top IEC clinical centrifuge)
               for 30 minutes using swing out buckets.

                   d. Blood should seperate out into three layers; 1. seru,.n/plasma, 2. Buffy
               coat 3.;Packed RBCS.


                   a. Re-move serum/plasma (top) layer to within a few mls of buffy coat at
               interface of plasma and red blood cells (RBCs), but do not disturb buffy coat.
               Save 13 mls of plasma/serum in 15 ml polypropylene screw top centrifuge tube
               (Corning 025319).

Document 4 f:/Week-34/BX005154/PREVENTIVE MEDICINE-ENVIRONMENTAL THREAT-USAEHA/appendix d sop collect of peripheral blood for :09139616151390
Control Fields 17
File Room = aug96_declassified
File Cabinet = Week-34
Box ID = BX005154
Unit = 22D SUPCOM
Parent Organization = ARCENT
Folder Title = PREVENTIVE MEDICINE-ENVIRONMENTAL THREAT-USAEHA
Folder Seq # = 71
Subject = APPENDIX D SOP COLLECT OF PERIPHERAL BLOOD FOR
Document Seq # = 90
Document Date =
Scan Date = 30-AUG-1996
Queued for Declassification = 01-JAN-1980
Short Term Referral = 01-JAN-1980
Long Term Referral = 01-JAN-1980
Permanent Referral = 01-JAN-1980
Non-Health Related Document = 01-JAN-1980
Declassified = 13-SEP-1996